GENIO italiano Giuseppe Cotellessa

  Rapid and Efficient Live Zebrafish Embryo Genotyping /   Genotipizzazione  rapida ed efficiente  di embrioni di pesci zebra vivi  Segnalato dal Dott. Giuseppe Cotellessa /   Reported by Dr. Giuseppe Cotellessa Fig. 1 Rapid and efficient live zebrafish embryo genotyping.  (A)  The step procedure for the method.  (B)  Representative image of genotyped zebrafish larva at 7 dpf. Lateral view, head to left.  (C)  Representative PCR results. dpf, days postfertilization; NC, negative control; PC, positive control; PCR, polymerase chain reaction. /   Genotipizzazione   rapida ed efficiente  di embrioni di zebrafish dal vivo . (A) La procedura passo per il metodo. (B) Immagine rappresentativa della larva di zebrafish genotipizzata a 7 dpf. Vista laterale, testa a sinistra. (C) risultati PCR rappresentativi. dpf, giorni di post fertilizzazione; NC, controllo negativo; PC, controllo positivo; PCR, reazione a catena della polimerasi. Abstract Most current methods for genotyping zebrafish embryo

 Atlas of Malaria Mosquitoes’ Immune System Assembled /   Atlante del Sistema immunitario delle zanzare della malaria assemblato Segnalato dal Dott. Giuseppe Cotellessa /  Reported by Dr. Giuseppe Cotellessa An international team of scientists led by investigators at the Wellcome Sanger Institute and the NIH has created the first cell atlas of mosquito immune cells to understand how the insects fight malaria, as well as other mosquito-borne infections. The mosquito host is essential for the malaria parasite to complete its lifecycle, so any disruption would dramatically reduce the transmission of one of the world’s deadliest diseases. Findings from the new study—published recently in  Science  through an article titled “ Mosquito cellular immunity at single-cell resolution “—discussed the discovery of new types of mosquito immune cells, including a rare cell type that could be involved in limiting malaria infection. The authors also identified molecular pathways implicated in controll

Forcing Fat Cells to Burn Calories /   Costringere le cellule adipose a bruciare calorie Segnalato dal Dott. Giuseppe Cotellessa / Reported by Dr. Giuseppe Cotellessa Researchers uncover the molecular pathway in brown and beige fat cells allows them to burn off calories instead of storing them as fat.  /  I ricercatori scoprono che il percorso molecolare nelle cellule adipose marroni e beige consente loro di bruciare calorie invece di immagazzinarle come grasso.  [Cell] With beach season in full swing, many folks would like the idea of having lipid cells be switched on to burn calories instead of storing them as fat. And though the idea of being able to eat that extra hot dog at the summer picnic and still fit into that bathing suit is appealing, there are even greater medical implications for understanding the pathways that govern fat metabolism. Now, investigators at Dana-Farber Cancer Institute in collaboration with scientists at the University of California, Berkeley, have identifi

 Novel Diabetes Therapy Might Be Found in Protein Commonly Found Throughout the Body /  Una nuova terapia per il diabete potrebbe essere trovata nelle proteine comunemente presenti in tutto il corpo Segnalato dal Dott. Giuseppe Cotellessa / Reported by Dr. Giuseppe Cotellessa A protein that’s common throughout the body plays a key role in regulating glucose levels, according to new research conducted in the Cell Signal Unit at the Okinawa Institute of Science and Technology Graduate University (OIST) and Riken Center of Integrative Medical Sciences. CNOT3 was found to silence a set of genes that would otherwise cause insulin-producing cells to malfunction, which is related to the development of diabetes. “We know that defects in beta cells can lead to high levels of glucose in the blood and, eventually, diabetes,” said Dina Mostafa, former PhD student in the Cell Signal Unit and first author of the paper, “ Loss of β-cell identity and diabetic phenotype in mice caused by disruption of

 Immune Response Insights against Fungal Pathogen Candida Auris /  Analisi della risposta immunitaria contro il patogeno fungino Candida Auris Segnalato dal Dott. Giuseppe Cotellessa /  Reported by Dr. Giuseppe Cotellessa Candida auris  emerged as an important fungal pathogen in 2009, when the previously unknown fungus was discovered in the infected ear of a seventy-year-old Japanese woman. Where  C. auris  suddenly came from was not clear, but soon after that, different strains appeared all over the world. It turned out to be a persistent, difficult to control fungus. Since then, the fungus has spread across six continents as a causative microorganism of hospital-acquired infections. It remains challenging on many fronts, not only difficult to diagnose but also resistant to many commonly used antifungal drugs. Now, an international team led by researchers from Radboud University Medical Center in the Netherlands, has discovered that the human immune system recognizes the fungus well.