GENIO italiano Giuseppe Cotellessa

Lo studio identifica come le cellule staminali del sangue mantengono il loro destino /  Study identifies how blood stem cells maintain their fate Segnalato dal Dott. Giuseppe Cotellessa /  Reported by Dr. Giuseppe Cotellessa  L'immagine mostra le cellule del sangue del topo:  un mix di cellule staminali e progenitrici, neutrofili  differenziati e cellule dell'identità mista. / Image  shows mouse blood cells — a mix of stem and  progenitor cells, differentiated neutrophils and  mixed identity cells.  Credito: Meijuan Chen,  laboratorio Cheloufi, UC Riverside. La comprensione dei meccanismi molecolari che  specificano e mantengono le identità di oltre 200  tipi cellulari del corpo umano è probabilmente uno  dei problemi più fondamentali della biologia  molecolare e cellulare, con implicazioni critiche  per il trattamento delle malattie umane.  Al centro  del processo decisionale sul destino cellulare ci  sono le cellule staminali che risiedono all'interno di  ogni tessuto de

Innovative solutions for accurate western blot imaging /  Soluzioni innovative per un imaging western blot accurato Segnalato dal Dott. Giuseppe Cotellessa / Reported by Dr. Giuseppe Cotellessa Fig 1. Dendrobium nobile orchid imaged in the ImageQuantTM 800 system using different LED and filter combinations. The imager revealed how parts of the flower fluoresced under different excitation wavelengths, showing vein-like patterns. / Dendrobium nobile orchid ripreso nel sistema ImageQuantTM 800 utilizzando diverse combinazioni di LED e filtri. L'imager ha rivelato come parti del fiore emettessero fluorescenza a diverse lunghezze d'onda di eccitazione, mostrando schemi simili a vene Introduction  A western blot is a common analytical technique used to detect specific proteins within a sample, evaluate the size of a particular protein and/or to determine the level of protein expression. While the advent of X-ray film and digital imaging solutions (e.g., charge-coupled device (CCD) c

 PTM site localization and isomer differentiation of phosphorylated peptides /  Localizzazione del sito PTM e differenziazione isomerica di peptidi fosforilati Segnalato dal Dott. Giuseppe Cotellessa / Reported by Dr. Giuseppe Cotellessa Tunable electron activated dissociation (EAD) MS/MS using the SCIEX ZenoTOF 7600 system. Protein phosphorylation is an important post-translational modification (PTM) as it is involved in a large variety of dynamic cellular processes. However, PTM site localization and quantification of phosphopeptides by collision induced dissociation (CID) MS/MS can be challenging, and phosphopeptides can exhibit a partial neutral loss of the phospho group (-98 Th). Phospho-isomer differentiation and subsequent precise PTM site localization can be achieved by measuring isomerspecific ions containing the actual modification (direct evidence), or by measuring differentiating fragment ions that do not contain the modification (indirect evidence). Depending on the pepti

Why qPCR is the gold standard for COVID-19 testing /  Perché qPCR è il gold standard per i test COVID-19 Segnalato dal Dott. Giuseppe Cotellessa / Reported by Dr. Giuseppe Cotellessa Every day there’s a new headline about the best ways to test for SARS-CoV-2 virus, the virus that causes COVID-19. In the following Q&A, Joshua Trotta, Senior Director Business Development, discusses what you need to know about qPCR, the gold standard for COVID-19 testing. This is a follow-up to our “ How does a COVID-19 test work? ” interview with Joshua on Science with a Twist earlier this year. What is an RT-qPCR test? “PCR” is shorthand for “polymerase chain reaction,” a scientific methodology to make millions and billions of copies of a specific DNA sample, which for the past 25 years has been one of the most widely used techniques in molecular biology. The genetic material in SARS-CoV-2 virus is stored in RNA. In the case of COVID-19 testing, RNA from a SARS-CoV-2 sample is first converted to it